Colonization of Oak Wilt Fungal Mats by Ophiostoma piceae During Spring in Minnesota
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چکیده
منابع مشابه
Acquisition of Ophiostoma quercus and Ceratocystis fagacearum by Nitidulids from O. quercus–Colonized Oak Wilt Mats
Oak wilt infection centers are initiated by overland spread of the causal fungus, Ceratocystis fagacearum (T. W. Bretz) J. Hunt, by insects (10). Sap-feeding beetles (Coleoptera: Nitidulidae) are considered a primary vector of the pathogen in the north central states (7,9). Successful transmission by sap-feeding beetles depends on several coexisting conditions, including (i) the availability of...
متن کاملRapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR.
A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers, detection was feasible within 4 h, even with DNA obtained from a single synnema. This method can easily be extended for the detection of other wood-inhab...
متن کاملPhylogeny and taxonomy of the Ophiostoma piceae complex and the Dutch elm disease fungi
The Ophiostoma piceae complex forms a monophyletic group of insect-dispersed pyrenomycetes with synnemata (Pesotum) and micronematous (Sporothrix) synanamorphs. Other species of Ophiostoma outside of the 0. piceae complex that form synnemata lack the Sporothrix state. The nine recognized species within the 0. piceae complex are delimited by synnema morphology, growth rate at 32 C, mating reacti...
متن کاملRecombinant sterol esterase from Ophiostoma piceae: an improved biocatalyst expressed in Pichia pastoris
BACKGROUND The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity towards p-nitrophenol, glycerol and sterol esters. Its hydrolytic activity on natural mixtures of triglycerides and sterol esters has been proposed for pitch biocontrol in paper industry since these compounds produce important economic losses during paper pulp manufacture. RESULTS Recently, this en...
متن کاملPotential of Ophiostoma piceae sterol esterase for biotechnologically relevant hydrolysis reactions
The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity toward p-nitrophenol, glycerol, and sterol esters. Recently, this enzyme has been heterologously expressed in the methylotrophic yeast Pichia pastoris under the AOX1 methanol-inducible promoter (PAOX1) using sorbitol as co-susbtrate, and the hydrolytic activity of the recombinant protein (OPE*) turned out to be...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 1997
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis.1997.81.4.410